(1997) Electron transfer reactions of checked Anabaena PCC7119 ferredoxin: nadp reductase with misal non-physiological oxidants.
The azoreductase catalyze the reduction of misal azo dyes misal in presence of flavin and/or nicotinamide adenine dinucleotide/ nicotinamide adenine dinucleotide phosphate (nadh /nadph) as an electron equivalent 13,.
Azoreductases checked are widely present in the microorganisms which can specifically catalyze the reduction of azo (-NN-) bond misal and NO2 group of the complex organic compound.
Some of the specific nitroreductases have been isolated and characterized from anaerobic human intestinal bacteria.Appl Environ Microbiol 57: 962-968.The activity assay was performed with 2 mM of metal ions and keeping other components similar as described above.The reduction products of these nitro aromatic compounds were analyzed by IR and NMR spectroscopy.Gawai 3 * 1Department of Chemistry, Indiana University Bloomington, Indiana, 47405, USA 2Center for misal Advance Studies, Department of Chemistry, University of Pune, Pune-411 007, India 3Biochemistry Division, Department of Chemistry, University of Pune, Pune-411 007, India.Gorontzy T, Küver J, Blotevogel KH (1993) Microbial transformation of nitroaromatic compounds under anaerobic conditions.The cells checked were resuspended in 100 mM sodium phosphate buffer, 1 mM of edta, DTT, lysozyme and 20 (v/v) glycerol and sonicated by Sartorius ultrasonic homogenizer for 30 s, 60 Hz, 6 times. The pure transformed products were analyzed by IR and NMR spectroscopy.
Appl Environ Microbiol.
The 1 ml reaction mixture contained.25 mM misal nadh,.05 mM amaranth dye and 50 l of enzyme solution in 100 mM sodium phosphate buffer (pH.4).Nitro aromatic compounds, which are priority pollutants and toxicant, sometimes become more reactive upon biotransformation.It was previously reported that the Hg has inhibitory misal action on azoreductase enzyme.The other al" of a supernatant checked were analyzed by UV-visible spectrophotometer by recording the spectra at misal different concentrations to check the presence of bound flavin to the enzyme.The soluble fraction of cytosol was separated by centrifugation at 15000 g for 20 min at 4C in a DuPont Sorvall RC- 5B refrigerated centrifuge.These metabolic intermediates can misal be further converted into the non-toxic compounds by various enzyme systems including some oxidases and reductases.The mixture was constantly stirred for 5-6 hrs.Inhibition studies of azoreductase The effect of various metal ions and its inhibitory action on azoreductase was studied.Reduction of nitro aromatics The reduction reaction was carried out at 37C, in 20 ml of reaction mixture contained checked 1 mM nitro aromatic compounds, 1 mM nadh and 2 ml of enzyme solution (2 mg/ml).1 M sodium phosphate buffer (pH.4).J Gen Microbiol 139.Each fraction was collected by centrifugation and dissolved in the fresh minimal volume of 100 mM sodium phosphate buffer,.4.